ÖZET
Amaç:
Bu çalışmanın amacı, Türkiye’de sıtmanın ikincil vektörü olan Anopheles superpictus’ta knockdown direncinden sorumlu olan L1014F, L1014S, L1014C allellerinin ve asetilkolinesteraz duyarsızlığından sorumlu olan Ace-1 G119S allellerinin varlığını tespit etmektir.
Yöntemler:
Aydın, Denizli ve Muğla illerinden 20’şer adet Anopheles superpictus ergin dişileri toplanmıştır. Daha sonra knockdown direncinden sorumlu olan L1014F, L1014S ve L1014C allellerine ve Ace-1 G119S alleline özgü primerler tasarlanarak allele özgü polimeraz zincir reaksiyonu ile bu üç Anopheles superpictus popülasyonunda bu allellerin varlığı taranmıştır.
Bulgular:
Aydın, Muğla ve Denizli popülasyonlarında çok düşük oranda L1014S mutasyonuna rastlanmakla birlikte, popülasyonların hiçbirinde ne kdr L1014F ve L1014C; ne de Ace-1 G119S mutasyonuna rastlanmıştır.
Sonuç:
Bu çalışmanın sonucu olarak, ilk kez Anopheles superpictus Ege bölgesi popülasyonlarında kdr L1014S mutasyonu tespit edilmiştir.
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