ABSTRACT

The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultenous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.

Keywords: Theileria annulata, Theileria buffeli, multiplex PCR