ABSTRACT

Plasmodium, Isospora, Toxoplasma ,Babesia and Cryptosporidium are parasites which have a signifi cant role in human health. The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from protozoan. Classical methods are not adequate for the identifi cation of some protozoon; also, molecular methods have to be used in designating the distinctions between the species. In recent years, one of the molecular methods which has been used frequently in the identifi cation of protozoon is the technique of LAMP. With the aid of the LAMP technique, from which it is possible to obtain reliable outcomes without the contribution of technical skills and professional equipment, in constant temperature it is possible to have a great number of copies from the targeted DNA in a short period. The aim of this collation is to give information about the usage of the technique of LAMP in the identifi cation of the protozoa which are important for human health and the comparison between the technique of LAMP and other molecular methods.

Keywords: LAMP (Loop-Mediated Isothermal Amplifi cation), Protozoa, Apicomplexa, Polimerase Change Reaction (PCR)