ABSTRACT

Objective:

In this study, we attempted to identify new Echinococcus granulosus isolates in the North West provinces of Iran based on the mitochondrial cytochrome c oxidase subunit 1 (CO1) sequence.

Methods:

Twenty-nine hydatid cysts from sheep and goats were collected. Genomic DNAs were extracted, and a partial sequence of the CO1 gene was amplified. Polymerase chain reaction products were cloned and sequenced with M13 primers in both directions.

Results:

All Iranian isolates were located in G1 and G3 genotypes. For the first time, a new G1 haplotype in two Iranian isolates were identified.

Conclusion:

It seems that this new haplotype was transmitted from Jordan to Iran or vice versa. (Turkiye Parazitol Derg 2015; 39: 286-90)

Keywords: Echinococcus granulosus, Cytochrome c oxidase, Genotyping

İran Kökenli Echinococcus granulosus G1 Haplotiplerinin Sitokrom C Oksidaz (Alt Ünite 1) Dizilimine Dayalı Moleküler Çalışması

ABSTRACT

References