In vitro and in vivo Nematocidal Activity of Allium sativum and Tagetes erecta Extracts Against Haemonchus contortus
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Original Investigation
P: 260-264
December 2015

In vitro and in vivo Nematocidal Activity of Allium sativum and Tagetes erecta Extracts Against Haemonchus contortus

Turkiye Parazitol Derg 2015;39(4):260-264
1. Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP-México, Department of Helminthology, Jiutepec, Morelos, México
2. Centro de Investigación en Biodiversidad y Conservación CIByC, UAEM, Cuernavaca, Morelos, México
3. Centro de Investigación en Biotecnología, Control de Plagas Agrícolas y Pecuarias, Cuernavaca, Morelos, México
4. Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México
No information available.
No information available
Received Date: 10.09.2015
Accepted Date: 15.10.2015
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ABSTRACT

Objective:

In the Mexican ethno-medicine, a number of plants have shown a successful anthelmintic activity. This fact could be crucial to identify possible green anti-parasitic strategies against nematodes affecting animal production. This research evaluated the in vitro and in vivo nematocidal effects of two single and combined plant extracts: bulbs of Allium sativum (n-hexane) and flowers of Tagetes erecta (acetone). The in vivo assay evaluated the administration of extracts either individually or combined against Haemonchus contortus in experimentally infected gerbils.

Methods:

The in vitro larvicidal activity percentage (LAP) of A. sativum and T. erecta extracts against H. contortus (L3) was determined by means of individual and combined usage of the extracts. Similarly, the extracts were evaluated in terms of reduction in the parasitic population in gerbils infected with H. contortus by individual and combined usage.

Results:

The LAP at 40 mg/mL was 68% with A. sativum and 36.6% with T. erecta. The combination caused 83.3% mortality of parasites. The oral administration of A. sativum and T. erecta extracts at 40 mg/mL, caused 68.7% and 53.9% reduction of the parasitic burden, respectively. Meanwhile, the combined effect of both extracts shown 87.5% reduction.

Conclusion:

This study showed evidence about the effect of A. sativum and T. erecta plant extracts by means of individual and combined usage against H. contortus in in vitro and in vivo bioassays in artificially H. contortus-infected gerbils as a model. (Turkiye Parazitol Derg 2015; 39: 260-4)