ÖZET
Amaç:
Trichomoniasis, dünya çapında en yaygın cinsel yolla bulaşan protozoa enfeksiyonudur. Metronidazol yaygın olarak trichomoniasis tedavisi için tercih edilen ilaç olarak kabul edilir, ancak potansiyel yan etkileri göz önüne alındığında, Trichomonas vaginalis’e karşı alternatif ilaçlar olarak Quercus brantii ve Artemisia aucheri Boiss'in hidro-alkolik ekstraktlarının terapötik etkilerini değerlendirmeyi amaçladık.
Yöntemler:
Trofozoitler, 5x105 trofozoit/mL yoğunlukta TYI-S-33 ortamında kültürlendi. Daha sonra, farklı konsantrasyonlarda bitki ekstraktları (32, 64, 125, 250, 500 ve 1.000 μg/mL) ve metronidazol (16, 32, 64, 125, 250 ve 500 μg/mL) ile inkübe edildiler. pozitif kontrol. Her gözenekli plakadaki trofozoitlerin sayısı, tripan mavisi boyama kullanılarak 2, 4, 6, 24, 48 ve 72 saat sonra ölçüldü. Son olarak, parazitin canlılığı hayati metilen mavisi boyaması ile değerlendirildi.
Bulgular:
Q. brantii ve A. aucheri Boiss’in 125, 250, 500 ve 1.000 ug/mL konsantrasyonlardaki hidro-alkolik özleri, parazite karşı önemli etkinlik gösterdi. Bulgularımız, minimum etkili konsantrasyonların 125 μg/mL olduğunu ve Q. brantii ve A. aucheri Boiss’in hidro-alkolik ekstraktlarının, 48 ve 72 saatlik tedaviden sonra T. vaginalis’i etkili bir şekilde yok etme yeteneğine sahip olduğunu gösterdi.
Sonuç:
Bu çalışmanın bulguları, Q. brantii ve A. aucheri Boiss’in hidro-alkolik ekstraktının T. vaginalis’te ölüme neden olabileceğini göstermiştir. Bununla birlikte, T. vaginalis’in tedavisinde bu bitkilerin bileşenlerini değerlendirmek için daha fazla tamamlayıcı in vivo çalışmalara ihtiyaç vardır.
References
1
Fakhrieh-Kashan Z, Arbabi M, Delavari M, Mohebali M, Hooshyar H. Induction of apoptosis by alcoholic extract of combination Verbascum thapsus and Ginger officinale on Iranian isolate of Trichomonas vaginalis. Iran J Parasitol 2018; 13: 72-8.
2
Hezarjaribi HZ, Fakhar M, Shokri A, Teshnizi SH, Sadough A, Taghavi M. Trichomonas vaginalis infection among Iranian general population of women: a systematic review and meta-analysis. Parasitol Res 2015; 114: 1291-300.
3
Bachmann LH, Lewis I, Allen R, Schwebke JR, Leviton LC, Siegal HA, et al. Risk and prevalence of treatable sexually transmitted diseases at a Birmingham substance abuse treatment facility. Am J Public Health 2000; 90: 1615.
4
Miller G, Klausner J, Coates T, Meza R, Gaydos CA, Hardick J, et al. Assessment of a rapid antigen detection system for Trichomonas vaginalis infection. Clin Diagn Lab Immunol 2003; 10: 1157-8.
5
Mashburn J. Etiology, diagnosis, and management of vaginitis. J Midwifery Womens Health 2006; 51: 423-30.
6
Arbabi M, Delavari M, Fakhrieh-Kashan Z, Hooshyar H. Review of Trichomonas vaginalis in Iran, based on epidemiological situation. J Reprod Infertil 2018; 19: 82.
7
Kissinger P. Epidemiology and treatment of trichomoniasis. Curr Infect Dis Rep 2015; 17: 31.
8
Sutton M, Sternberg M, Koumans EH, McQuillan G, Berman S, Markowitz L. The prevalence of Trichomonas vaginalis infection among reproductive-age women in the United States, 2001-2004. Clin Infect Dis 2007; 45: 1319-26.
9
Sena AC, Miller WC, Hobbs MM, Schwebke JR, Leone PA, Swygard H, et al. Trichomonas vaginalis infection in male sexual partners: implications for diagnosis, treatment, and prevention. Clin Infect Dis 2007; 44: 13-22.
10
Ali V, Nozaki T. Current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by “amitochondriate” protozoan parasites. Clin Microbiol Rev 2007; 20: 164-87.
11
Arbabi M, Delavari M, Kashan ZF, Taghizadeh M, Hooshyar H. Ginger (Zingiber officinale) induces apoptosis in Trichomonas vaginalis in vitro. Int J Reprod Biomed 2016; 14: 691.
12
Schwebke JR, Barrientes FJ. Prevalence of Trichomonas vaginalis isolates with resistance to metronidazole and tinidazole. Antimicrob Agents Chemother 2006; 50: 4209-10.
13
F Kashan Z, Arbabi M, Delavari M, Hooshyar H, Taghizadeh M, Joneydy Z. Effect of Verbascum thapsus Ethanol Extract on Induction of Apoptosis in Trichomonas vaginalis in vitro. Infect Disord Drug Targets 2015; 15: 125-30.
14
Hashemi P, Abolghasemi M, Fakhari A, Ebrahimi SN, Ahmadi S. Hydrodistillation–solvent microextraction and GC–MS identification of volatile components of Artemisia aucheri. Chromatographia 2007; 66: 283-6.
15
Bahador N, Baserisalehi M. The effect of Quercus castaneifolia extract on pathogenic enteric bacteria. Anaerobe 2011; 17: 358-60.
16
Taib M, Rezzak Y, Bouyazza L, Lyoussi B. Medicinal uses, phytochemistry, and pharmacological activities of Quercus Species. Evid Based Complement Alternat Med 2020; 2020: 1920683.
17
Akram E, Masoud K, Vahid N. Evaluation of the antibacterial and wound healing activity of Quercus persica. Journal of Basic & Applied Sciences 2012; 8: 118-23.
18
Safary A, Motamedi H, Maleki S, Seyyednejad S. A preliminary study on the antibacterial activity of Quercus brantii against bacterial pathogens, particularly enteric pathogens. Int J Botany 2009; 5: 176-80.
19
Kooti W, Ghasemiboroon M, Asadi-Samani M, Ahangarpoor A, Noori Ahmad Abadi M, Afrisham R, et al. The effects of hydro-alcoholic extract of celery on lipid profile of rats fed a high fat diet. Advances in Environmental Biology 2014; 8: 325-30.
20
Fakhrie KZ, Arbabi M, Delavari M, Taghizadeh M, Hooshyar H, Solaymani F. The effect of aqueous and alcoholic extracts of Pelarqonium roseum on the growth of Trichomonas vaginalis in vitro. Feyz 2014; 18: 369-75.
21
Durel P, Couture J, Collart P, Girot C. Flagyl (metronidazole). Br J Vener Dis 1960; 36: 154.
22
Aminou HA, Alam-Eldin YH, Hashem HA. Effect of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin on the ultrastructure of Trichomonas vaginalis trophozoites. J Parasi Dis 2016; 40: 707-13.
23
Tagboto S, Townson S. Antiparasitic properties of medicinal plants and other naturally occurring products. Adv Parasitol 2001; 50: 199-295.
24
Youse HA, Kazemian A, Sereshti M, Rahmanikhoh E, Ahmadinia E, Rafaian M, et al. Effect of Echinophora platyloba, Stachys lavandulifolia, and Eucalyptus camaldulensis plants on Trichomonas vaginalis growth in vitro. Adv Biomed Res 2012; 1: 79.
25
Hassani S, Asghari G, Yousefi H, Kazemian A, Rafieiean M, Darani HY. Effects of different extracts of Eucalyptus camaldulensis on Trichomonas vaginalis parasite in culture medium. Adv Biomed Res 2013; 2: 47.
26
Simbar M, Azarbad Z, Mojab F, Majd HA. A comparative study of the therapeutic effects of the Zataria multiflora vaginal cream and metronidazole vaginal gel on bacterial vaginosis. Phytomedicine 2008; 15: 1025-31.
27
Iwalewa EO, Omisore N, Adewunmi CO, Gbolade A, Ademowo O, Nneji C, et al. Anti-protozoan activities of Harungana madagascariensis stem bark extract on trichomonads and malaria. J Ethnopharmacol 2008; 117: 507-11.
28
Frasson AP, dos Santos O, Duarte M, da Silva Trentin D, Giordani RB, da Silva AG, et al. First report of anti-Trichomonas vaginalis activity of the medicinal plant Polygala decumbens from the Brazilian semi-arid region, Caatinga. Parasitol Res 2012; 110: 2581-7.
29
Gokmen AA, Can H, Kayalar H, Pektaş B, Kaya S. In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium. J Infect Dev Ctries 2019; 13: 240-4.
30
Berköz M, Yıldoırım M, Krośniak M, Francik R, Allahverdiyev O. Antioxidant Activity Of Different Extracts From Stalk Part Of Rhub Arb (Rheum Rhabarbarum L.). Van Sag Bil Derg 2018; 11: 11-5.
31
Mojarrab M, Shiravand A, Delazar A, Heshmati Afshar F. Evaluation of in vitro antimalarial activity of different extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and fractions of the most potent extracts. ScientificWorldJournal 2014; 2014: 825370.
32
Sharif M, Ziaei H, Azadbakht M, Daryani A, Ebadattalab A, Rostami M. Effect of methanolic extracts of Artemisia aucheri and Camellia sinensis on Leishmania major (in vitro). Turkish Journal of Medical Sciences 2007; 36: 365-9.
33
Rakić S, Petrović S, Kukić J, Jadranin M, Tešević V, Povrenović D, et al. Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia. Food Chemistry 2007; 104: 830-4.
34
Aleebrahim-Dehkordy E, Rafieian-Kopaei M, Amini-Khoei H, Abbasi S. In Vitro Evaluation of Antioxidant Activity and Antibacterial Effects and Measurement of Total Phenolic and Flavonoid Contents of Quercus brantii L. Fruit Extract. J Diet Suppl 2019; 16: 408-16.
