ÖZET
Amaç:
Mevcut araştırmanın amacı, deniz ascidian tunikatlarından izole edilen bileşiklerin Trichomonas vajinalis üzerindeki antiparazit etkilerini değerlendirmektir.
Yöntemler:
Ascidian tunikatlar toplandıktan sonra küçük parçalar halinde kesildi, dondurularak kurutuldu ve toz haline getirildi. Nihai malzeme, çift damıtılmış su, etanol, n-heksan ve diklorometan içerisinde ekstraksiyona tabi tutuldu. Ekstraktları parçalara ayırmak ve en biyoaktif bileşiği belirlemek için silika jel kolon kromatografisi ve GC-M/S analizi kullanıldı.
Bulgular:
Etanol ekstraktının silika jel kolon kromatografisinin 18. fraksiyonu T. vajinalis’e karşı en etkili olanıydı. Fraksiyon 18 için ilgili IC50, CC50 ve SI değerleri 28,62 μg/mL, ˃800 μg/mL ve ˃27,95 idi. Bu fraksiyonun GC-M/S analizi, vero hücrelerine karşı toksisitesi yalnızca %10,15 olan önemli bir fenolik bileşiği (2,4-bis (1,1-dimetil etil) tanımladı.
Sonuç:
T. vajinalis üzerinde güçlü öldürücü etkiye sahip olan fenol-2,4-bis (1,1-dimetiletil) içeren etanolik fraksiyon antiparazit ilaç adayı olarak değerlendirilebilir.
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