ÖZ
Amaç
Avrupa, Asya ve Kuzey Afrika’da bulunan bir sivrisinek türü olan Culiseta longiareolata (Cs. longiareolata), bruselloz, kuş gribi, Batı Nil ensefaliti ve sıtma gibi çeşitli hastalıklar için önemli bir vektördür. Bu çalışma, Güney İran’ın Fars eyaletindeki Cs. longiareolata sivrisinek popülasyonunun genetik çeşitliliğini değerlendirmeyi amaçlamıştır.
Yöntemler
Bu çalışmada, İran’ın Fars eyaletindeki 12 lokasyondan daldırma yöntemi kullanılarak Cs. longiareolata sivrisinek örnekleri toplandı. Yetişkin sivrisinekler aspiratörler ve sivrisinek ağları kullanılarak toplandı ve daha sonra DNA ekstraksiyonu için -20 °C’de saklandı. Genetik analiz için COI geni polimeraz zincir reaksiyonu (PCR) yoluyla çoğaltıldı. Dizileme işlemi Güney Kore’deki Bioneer Şirketi tarafından yapıldı ve sonuçlar Chromas, ClustalW2 ve MEGA7 gibi biyoinformatik araçlar kullanılarak analiz edildi. Nihai diziler daha ileri analizler için GenBank’a kaydedildi.
Bulgular
Maksimum olabilirlik yöntemi kullanılarak yapılan filogenetik analiz, İran’dan alınan Cs. longiareolata örneklerinin çeşitli ülkelerden gelen popülasyonlarla kümelendiğini ortaya koydu. Analiz, Temura-Nei modeli kullanılarak 504 pozisyonlu 17 nükleotid dizisini içeriyordu. Altı haplotip ve 0,721 haplotip çeşitliliği ile genetik çeşitlilik indeksi 0,0165 idi. Demografik genişleme indeksleri, popülasyonda potansiyel bir genişleme olayına işaret etti. Çalışma için toplam 274 sivrisinek toplandı, 18’i PCR ile incelendi ve 6’sı dizilendi.
Sonuç
Bu çalışma, İran’daki Cs. longiareolata popülasyonlarının küresel popülasyonlarla genetik olarak bağlantılı olduğunu ortaya koymuştur. Araştırma, sivrisinek dinamiklerini anlamada ve hastalık kontrol çabalarını geliştirmede genetik çeşitliliğin önemini vurgulamaktadır. Vektör kaynaklı hastalıkların yönetimi için devam eden genetik izleme şarttır.
Anahtar Kelimeler:
Culiseta longiareolata, genetik çeşitlilik, COI geni, filogenetik analiz, vektör kaynaklı hastalıklar
Kaynaklar
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