Öz
Amaç
Acanthamoeba’nın ilaç direnci, alternatif yeni yöntem bulma konusunda önemli bir endişe kaynağıdır. Bu araştırmada kimyasal veya bitkisel ilaçlar ile mikrodalga radyasyonunun kombine tedavisinden faydalanılmaya çalışılmıştır.
Yöntemler
Acanthamoeba, besleyici olmayan agar üzerinde yetiştirildi. Siyah çay ekstraktları maserasyon yöntemiyle hazırlandı. Mevcut çalışmada kullanılan gentamisinin nihai konsantrasyonları 0,75 ve 0,375 mg/mL’dir. Örnekler ilaç inkübasyon süresi ve inkübasyon öncesi ve sonrası radyasyona tekrar maruz kalma (24, 48 ve 72 saat) açısından 12 gruba ayrıldı. Gentamisin bileşiği ve siyah çay ekstraktlarının mikrodalgaya maruz bırakılmasıyla parazit üzerindeki etkileri değerlendirildi.
Bulgular
Mevcut deneyler, gentamisin, siyah çay ve radyasyon tedavisi grubuyla karşılaştırıldığında bileşik tedavi gruplarında parazitin büyüme inhibisyonunun önemli ölçüde daha yüksek olduğunu gösterdi (p<0,0001-p<0,04). Mikrodalga radyasyonunun 72 saat içinde büyüme inhibisyonunun artan bir eğilime yol açtığı görülmektedir.
Sonuç
Mikrodalga radyasyonu, Acanthamoeba kistlerinin gentamisin ve siyah çay ekstraktları ile tedavisinde önemli bir tamamlayıcı rol oynayabilir. Bu etkinin gentamisin ile inkübe edilen ışınlanmış kistlerde daha belirgin olduğu ve inkübasyon süresinin artmasına ve radyasyon tekrarına da bağlı olduğu görüldü.
Anahtar Kelimeler: Acanthamoeba, siyah çay, kombinasyon tedavisi, ilaç direnci, gentamisin, radyo frekansı
Kaynakça
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