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Characterisation of the <i>Leishmania donovani/L. infantum</i> Hybrid Isolated from an Autochothonous Kala-Azar Patient: Preliminary Results of an In Vivo Model
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Original Investigation
VOLUME: 45 ISSUE: 2
P: 95-100
June 2021

Characterisation of the Leishmania donovani/L. infantum Hybrid Isolated from an Autochothonous Kala-Azar Patient: Preliminary Results of an In Vivo Model

Turkiye Parazitol Derg 2021;45(2):95-100
DOI: 10.4274/tpd.galenos.2021.14622
Özgür Kurt 1
Nesteren Mansur Özen 2
Elif Merve Aydın 2
Deniz Ece Kaya 3
Cavit Kerem Kayhan 4
Sinem Öktem Okullu 1
Ümit İnce 4,5
Fadile Yıldız Zeyrek 6
1. Acıbadem Mehmet Ali Aydınlar Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
2. Acıbadem Mehmet Ali Aydınlar Üniversitesi Sağlık Bilimleri Enstitüsü, Medikal Biyoteknoloji Anabilim Dalı, İstanbul, Türkiye
3. Acıbadem Mehmet Ali Aydınlar Üniversitesi Sağlık Bilimleri Enstitüsü, Biyoistatistik ve Biyoinformatik Anabilim Dalı, İstanbul, Türkiye
4. Acıbadem Sağlık Grubu, Maslak Hastanesi, Patoloji Kliniği, İstanbul, Türkiye
5. Acıbadem Mehmet Ali Aydınlar Üniversitesi Tıp Fakültesi, Tıbbi Patoloji Anabilim Dalı, İstanbul, Türkiye
6. Harran Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Şanlıurfa, Türkiye
No information available.
No information available
Received Date: 08.02.2021
Accepted Date: 20.04.2021
Publish Date: 07.06.2021
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ABSTRACT

Objective:

In the present study, preliminary outcomes of the in vivo assessment of a Leishmania donovani/L. infantum hybrid isolated from a hospitalised patient with visceral leishmaniasis in Manisa and identified through analysis of the Leishmania-specific ITS-1, hsp70 and cpb gene regions are presented in comparison with reference strains of L. donovani and L. infantum.

Methods:

Three different study groups [(SG); n=16 mice each] and a control group (n=8 mice) were established with female Balb/C mice weighing 25-30 g. Reference L. donovani (MHOM/IN/1980/DD8), reference L. infantum (MHOM/TN/1980/IP1) and a L. donovani/L. infantum hybrid (MHOM/TR/2014/CBVL-LI/ LD), stored in liquid nitrogen, were thawed, cultured and incubated at 25 °C. A 15-μL dose of 1x108/mL promastigotes of three strains was applied to the tail veins of mice in the SG. After the mice were sacrificed, the liver and spleen tissues were removed and stored for immunological, immunohistochemical and pathological analyses.

Results:

The presence of infection in the liver and spleen tissues of mice was detected both by a specific enzyme-linked immunosorbent assay test and from the recovery of Leishmania promastigotes from liver and spleen tissues in NNN medium. However, Leishmania amastigotes were not observed in the touch biopsy smears of livers or spleens in either of the SGs. In addition, no evidence of tissue damage was identified in the SGs after immunohistochemical staining (with antibodies against IL-9, CD-117, MBP, CD163, CD4, CD8 and CD31).

Conclusion:

The obtained results show that hybrid Leishmania and reference L. donovani and L. infantum strains reached the liver and spleens of Balb/C mice in SGs but were of no pathological consequence. Yet, these three Leishmania isolates caused skin lesions when applied subcutaneously in Balb/C mice in another study. The findings presented in this study will be reassessed upon completion of the project, once the final results are obtained.

Keywords: Leishmania sp., hybrid, Kala-Azar, Turkey, animal model

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