ABSTRACT

Objective:

This study was carried out to detect house dust mites in houses and to investigate group 1 antigens of Dermatophagoid species in Ordu, Giresun, Trabzon and Rize provinces of the Central and Eastern Black Sea Region.

Methods:

Dust samples obtained from the beds were subjected to both microscopic and antigenic examination. Samples prepared by the lactic acid method for microscopic examination were evaluated under a light microscope. Antigenic analysis was performed by investigating Der p 1 and Der f 1 belonging to D. pteronyssinus and D. farinae by ELISA test.

Results:

90.3% of the dust samples were evaluated positive by microscopic examination (10x, 40x) and 149 mites were detected. D. pteronyssinus 74%, D. farinae 13%, Dermatophagoides spp. growth forms 5%, Cheyletus spp. 1%, E. maynei 1%, C. arcuatus 1%, T. putrescentiae 1%, L. destructor 1% and unidentified mites were detected at the rate of 3% respectively. Der p 1 antigen was detected in 93% and Der f 1 antigen in 84.7%. The highest amount of antigen detected in one gram of powder was 1,272 μg for Der p 1 and 0,482 μg for Der f 1.

Conclusion:

No difference was observed between mite species and distribution in the provinces where the study was conducted (p<0.05). Dermatophagoides were found in 93% of the population. The low (4%) rate of storage/food mites is related to the fact that samples were not taken from the floors. Antigen accumulation may be important in the beds since the activity of the mites is observed throughout the year in temperate and humid regions. It is thought that this diagnosis method can be used and can be taken into account in terms of the environments in which sensitive people live.

Keywords: D. pteronyssinus, D. farinae, Der p 1, Der f 1, Black Sea Region

References

Colloff MJ. Dust Mites. Csiro Publishing. Csiro Publishing; 2009. p.125-214.

Krantz G, Walter DE. A manual of acarology. Texas Tech University Press. 3rd Edition. 2009.

Aycan OM, Atambay M, Daldal UN. Ev tozu akarlarinin görülme durumunun sosyal değişkenler açisindan incelenmesi [Investigation of house dust mite incidence related to social factors]. Turkiye Parazitol Derg 2007; 31: 219-24.

Liu Z, Bai Y, Ji K, Liu X, Cai C, Yu H, et al. Detection of Dermatophagoides farinae in the dust of air conditioning filters. Int Arch Allergy Immunol 2007; 144: 85-90.

Podder S, Biswas H, Kumar Saha G. A faunistic survey of house dust mites of Kolkata, West Bengal, India. Acarological Studies 2021; 3: 22-31.

Fernández-Caldas E, Puerta L, Caraballo L. Mites and allergy. Chem Immunol Allergy 2014; 100: 234-42.

Cohen S. The allergy archives. The history of the finding of the house dust mite. J Allergy Clin Immunol 2004; 113: 573-6.

Voorhorst R, Spieksma F, Varekamp H. The house-dust mite (Dermatophagoides pteronyssinus) and the allergens it produces. Identity with the house-dust allergen. J Allergy 1967; 39: 325-39.

Bessot JC, Pauli G. Mite allergens: an overview. Eur Ann Allergy Clin Immunol 2011; 43: 141-56.

Cui Y, Wang Q, Jia H. Consideration of methods for identifying mite allergens. Clin Transl Allergy 2018; 8: 14.

Kort HS, Kniest FM. Four-year stability of Der p I in house dust under simulated domestic conditions in vitro. Allergy 1994; 49: 131-3.

Suesirisawad S, Malainual N, Tungtrongchitr A, Chatchatee P, Suratannon N, Ngamphaiboon J. Dust mite infestation in cooking flour: experimental observations and practical recommendations. Asian Pac J Allergy Immunol 2015; 33: 123-8.

Tovey ER, Chapman MD, Platts-Mills TA. Mite faeces are a major source of house dust allergens. Nature 1981; 289: 592-3.

Arlian LG, Morgan MS, Neal JS. Dust mite allergens: ecology and distribution. Curr Allergy Asthma Rep 2002; 2: 401-11.

Casas L, Sunyer J, Tischer C, Gehring U, Wickman M, Garcia-Esteban R, et al. Early-life house dust mite allergens, childhood mite sensitization, and respiratory outcomes. Allergy 2015; 70: 820-7.

Celebioglu E, Ozturk AB, Comert S, Karakaya G, Kalyoncu AF. Storage mite sensitisation is associated with early life village residence in Turkey. Allergol Immunopathol (Madr) 2013; 41: 402-6.

Colloff MJ, Spieksma FT. Pictorial keys for the identification of domestic mites. Clin Exp Allergy 1992; 22: 823-30.

Colloff MJ. Taxonomy and identification of dust mites. Allergy 1998; 53: 7-12.

Hart BJ, Fain A. Morphological and biological studies of medically important house-dust mites. Acarologia 1988; 285-96.

Cevızcı S, Gökçe S, Bostan K, Kaypmaz A. Depo gıdalarını ve peynirleri enfeste eden akarlara halk sağlığı açısından bakış [A view of mites infestation on cheese and stored foods in terms of public health]. Turkiye Parazitol Derg 2010; 34: 191-9.

Reboux G, Valot B, Rocchi S, Scherer E, Roussel S, Millon L. Storage mite concentrations are underestimated compared to house dust mite concentrations. Exp Appl Acarol 2019; 77: 511-25.

Mutlu D, Akdemir C, Uzunoğlu E, Direkel Ş, Cebeci Güler N. The Investigation of the Prevalance and Epidemiology of House Dust Mites in Giresun. Turkiye Parazitol Derg 2019; 43: 78-82.

Zeytun E, Doğan S, Ünver E, Özçiçek F. Correction: Evaluation of Dermatophagoides pteronyssinus (Trouessart) and D. farinae Hughes (Acari: Pyroglyphidae) sensitivity in patients with allergic rhinitis: a comparative study. Syst Appl Acarol 2018; 23: 404.

Zeytun E, Doğan S, Özçiçek F, Ünver E, Dilkaraoğlu S. Comparison of Living and Bedrooms in Terms of House Dust Mites in the Province of Erzincan, Turkey. J Med Entomol 2016; 53: 26-30.

Çelik N. Samsun ilinde ev tozu akarları türlerinin belirlenmesi ve alerjik astım ile arasındaki ilişkinin ortaya konulması. 19 Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi. 2009.

Kılınçarslan L. Kayseri’de Ev Tozu Akarlarının Yayılışı, Ankara Üniversitesi, Sağlık Bilimleri Enstitüsü, (Yüksek Lisans Tezi). 2012.

Akdemır C, Gürdal H. Kütahya’da ev tozu akarları [House dust mite in Kutahya, Turkey.]. Turkiye Parazitol Derg 2005; 29: 110-5.

Aykut M, Köksal Ö, Doğan S, Ayyildiz N. House dust mites of Bitlis and Muş provinces. Türk Entomol Bült 2013; 3: 169-77.

Aykut M, Erman OK, Doğan S. Seasonal changes of house dust mite population in Bitlis and Muş provinces of Turkey. Turkiye Parazitol Derg 2013; 37: 113-7.

Zeytun E, Doğan S, Aykut M, Özçiçek F, Ünver E, Özçiçek A. House Dust Mites in Erzincan Province. Turkiye Parazitol Derg 2015; 39: 124-30.

Güleğen E, Gırışgın O, Kütükoğlu F, Gırışgın AO, Coşkun SZ. Bursa evlerinde bulunan ev tozu akar türleri [Mite species found in house dust in houses in Bursa.]. Turkiye Parazitol Derg 2005; 29: 185-7.

Aygan Ç, Özçelik S. Sivas yöresinde ev tozu akarlarının yaygınlığı ve atopik alerjideki rolü. Turkiye Parazitol Derg 2002; 26: 186-91.

Ataş AD, Baysal Bakay B, Bakay H, Gülpınar DG. Factors Affecting the Prevalence of House Dust Mite in Tekirdag and Istanbul Provinces in Comparison with House Dust Mite Population of Sivas Province During the Same Period. Turkiye Parazitol Derg 2021; 45: 137-45.

Akyazi R, Soysal M, Klimov PB, Altunç YE. House dust mite species in Ordu province, Turkey. J Agri Sci 2019; 25: 417-26.

Kalpaklioğlu AF, Emekçi M, Ferizli A, Misirligil Z; House-Dust Mite Working Group. A survey of acarofauna in Turkey: comparison of seven different geographic regions. Allergy Asthma Proc 2004; 25: 185-90.

Aykut M, Yilmaz H. Muş’un Hasköy ılçesinde ev tozu akarlarının yayılışı [Distribution of house dust mites in Hasköy town, Muş]. Turkiye Parazitol Derg 2010; 34: 160-3.

Gulkan B, Degerli S, G Culha, Savas N. Search of house mite’s fauna and investigation of relationship between house dust mite and allergy in the province of Hatay, Turkey. Kuwait Medical J 2019; 51: 78-85.

Sánchez-Borges M, Fernandez-Caldas E, Thomas WR, Chapman MD, Lee BW, Caraballo L, et al. International consensus (ICON) on: clinical consequences of mite hypersensitivity, a global problem. World Allergy Organ J 2017; 10: 14.

Demirtaş N, Ceylan E, Kırdar S, Karadağ F, Çildağ O. The Effect of Indoor Environmental Characteristics on the Detection of House Dust Mite Der p2 and Der f2 in Asthmatics. Meandros Med Dent J 2016; 17: 129-66.

Gulbahar O, Mete N, Kokuludag A, Sin A, Sebik F. House dust mite allergens in Turkish homes. Allergy 2004; 59: 231.

Prevalence of House Dust Mites and Presence of Der p 1 and Der f 1 in Ordu, Giresun, Trabzon and Rize Provinces

ABSTRACT

References