Fixation, Staining and Preparation of Permanent Mounts of Helminths
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Review
P: 177-181
June 2008

Fixation, Staining and Preparation of Permanent Mounts of Helminths

Turkiye Parazitol Derg 2008;32(2):177-181
1. Harran Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Şanlıurfa, Türkiye
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Received Date: 02.11.2007
Accepted Date: 06.03.2008
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ABSTRACT

The techniques for the collection, relaxation, preservation and staining of helminths are very important for parasitologists. Parasites should be collected alive and fixed directly in the living condition. These procedures insure proper preservation of internal and external details of parasites. There are various methods for relaxing and preserving the normal morphology of helminths. These methods are absolutely essential for permanent preservation of the specimens. Staining and mounting techniques vary depending upon size of specimens, species, and stage of development of the organisms. In this review, the preparation of permanent mounts, relaxation, fixation and staining methods of helminths has been discussed.

Keywords:
Helminth
relaxation
fixation
staining
permanent mounts